Genome Analysis A Comparative Genomics Strategy for Targeted Discovery of Single-Nucleotide Polymorphisms and Conserved-Noncoding Sequences in Orphan Crops
نویسندگان
چکیده
Plant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602 (F.A.F., H.P.S., H.C.L., S.R.S., A.H.P.); Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad 224264, Uttar Pradesh, India (H.P.S.); University of Agricultural Sciences, Krishinagar, Dharwad 580005, India (H.C.L.); and Department of Plant Sciences, University of Colombo, Colombo 03, Sri Lanka (T.D.S.)
منابع مشابه
A comparative genomics strategy for targeted discovery of single-nucleotide polymorphisms and conserved-noncoding sequences in orphan crops.
Completed genome sequences provide templates for the design of genome analysis tools in orphan species lacking sequence information. To demonstrate this principle, we designed 384 PCR primer pairs to conserved exonic regions flanking introns, using Sorghum/Pennisetum expressed sequence tag alignments to the Oryza genome. Conserved-intron scanning primers (CISPs) amplified single-copy loci at 37...
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